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mouse primary cd8 t cells  (Miltenyi Biotec)


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    Miltenyi Biotec mouse primary cd8 t cells
    Mouse Primary Cd8 T Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 638 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+primary+cd8+t+cells/CD8a%2B+T+Cell+Isolation+Kit%2C+mouse/pm40780215-376-0-22
    Average 97 stars, based on 638 article reviews
    mouse primary cd8 t cells - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Isolation:

    Article Title: TGFβ suppresses CD8 + T cell expression of CXCR3 and tumor trafficking
    Article Snippet: .. Mouse primary CD8 + T cells were isolated and purified from spleens by magnetic negative selection using a mouse CD8α + T cell isolation kit (Miltneyi Biotec). ..

    Article Title: CHI3L3 + immature neutrophils inhibit anti-tumor immunity and impede immune checkpoint blockade therapy in bone metastases.
    Article Snippet: Article CHI3L3 + immature neutrophils inhibit anti-tumor immunity and impede immune checkpoint blockade therapy in bone metastases

    Article Title: Perforin Gene Transfer Into Hematopoietic Stem Cells Improves Immune Dysregulation in Murine Models of Perforin Deficiency
    Article Snippet: The cells were cultured in RPMI medium with 10% fetal calf serum, 1% Pen/Strep, 1 mmol/l 2-mercaptoethanol (Invitrogen), 1 mmol/l sodium pyruvate (Invitrogen), and 1000 U/ml IL-2 (Peprotech, Rocky Hill, NJ). .. Mouse primary CD8 + T cells were isolated from spleens of prf −/− mice using CD8a (Ly-2) microbeads (Miltenyi Biotec) following the manufacturer's instructions. .. The cells were cultured in RPMI medium with 10% fetal calf serum, 1% Pen/Strep, 1 mmol/l 2-mercaptoethanol, 1 mmol/l sodium pyruvate, 30 U/ml IL-2, and 1 μg/ml anti-CD3 antibody (BD Biosciences, Oxford, UK).

    Purification:

    Article Title: TGFβ suppresses CD8 + T cell expression of CXCR3 and tumor trafficking
    Article Snippet: .. Mouse primary CD8 + T cells were isolated and purified from spleens by magnetic negative selection using a mouse CD8α + T cell isolation kit (Miltneyi Biotec). ..

    Selection:

    Article Title: TGFβ suppresses CD8 + T cell expression of CXCR3 and tumor trafficking
    Article Snippet: .. Mouse primary CD8 + T cells were isolated and purified from spleens by magnetic negative selection using a mouse CD8α + T cell isolation kit (Miltneyi Biotec). ..

    Article Title: CD47-Dependent Regulation of Immune Checkpoint Gene Expression and MYCN mRNA Splicing in Murine CD8 and Jurkat T Cells
    Article Snippet: .. Mouse primary CD8 + T cells were obtained from mouse spleens by negative selection, as described previously [ , ], using the mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec, Gaithersburg, MD, USA). ..

    Cell Isolation:

    Article Title: TGFβ suppresses CD8 + T cell expression of CXCR3 and tumor trafficking
    Article Snippet: .. Mouse primary CD8 + T cells were isolated and purified from spleens by magnetic negative selection using a mouse CD8α + T cell isolation kit (Miltneyi Biotec). ..

    Article Title: CD47-Dependent Regulation of Immune Checkpoint Gene Expression and MYCN mRNA Splicing in Murine CD8 and Jurkat T Cells
    Article Snippet: .. Mouse primary CD8 + T cells were obtained from mouse spleens by negative selection, as described previously [ , ], using the mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec, Gaithersburg, MD, USA). ..



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    D-mannose synergizes with PD-1 antibody to inhibit TNBC growth. ( A ) Schematic representation of the animal experiment process. ( B ) Representative tumors of 4T1 cells in BALB/c mice treated with D-mannose or/and anti–PD-1 antibody. ( C ) Tumor growth of 4T1 cells in BALB/c mice following treatment with D-mannose or/and anti–PD-1 antibody was determined. Tumors were measured at the indicated time points. n = 8 mice per group. Statistical differences were determined by ordinary one-way ANOVA. ns, no significance, **** P < 0.0001. ( D ) Kaplan–Meier survival curves for mice injected with 4T1 cells and treated with D-mannose or/and anti–PD-1 antibody. The P value, comparing every two groups, was determined using log-rank test and is shown in the table Below the figure. ( E ) Western blot analysis of PD-L1 level in 4T1 tumor tissues as indicated. ( F ) Immunohistochemistry showing CD8 + T cell infiltration and granzyme B expression in the 4T1 tumor tissues as indicated (Scale bars, 100 μm). ( G ) Quantifications of images in ( F ). Data represent mean ± SD from six independent samples of each group. Statistical differences were determined by ordinary one-way ANOVA. ns, no significance, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
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    Image Search Results


    D-mannose synergizes with PD-1 antibody to inhibit TNBC growth. ( A ) Schematic representation of the animal experiment process. ( B ) Representative tumors of 4T1 cells in BALB/c mice treated with D-mannose or/and anti–PD-1 antibody. ( C ) Tumor growth of 4T1 cells in BALB/c mice following treatment with D-mannose or/and anti–PD-1 antibody was determined. Tumors were measured at the indicated time points. n = 8 mice per group. Statistical differences were determined by ordinary one-way ANOVA. ns, no significance, **** P < 0.0001. ( D ) Kaplan–Meier survival curves for mice injected with 4T1 cells and treated with D-mannose or/and anti–PD-1 antibody. The P value, comparing every two groups, was determined using log-rank test and is shown in the table Below the figure. ( E ) Western blot analysis of PD-L1 level in 4T1 tumor tissues as indicated. ( F ) Immunohistochemistry showing CD8 + T cell infiltration and granzyme B expression in the 4T1 tumor tissues as indicated (Scale bars, 100 μm). ( G ) Quantifications of images in ( F ). Data represent mean ± SD from six independent samples of each group. Statistical differences were determined by ordinary one-way ANOVA. ns, no significance, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

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    Figure Lengend Snippet: D-mannose synergizes with PD-1 antibody to inhibit TNBC growth. ( A ) Schematic representation of the animal experiment process. ( B ) Representative tumors of 4T1 cells in BALB/c mice treated with D-mannose or/and anti–PD-1 antibody. ( C ) Tumor growth of 4T1 cells in BALB/c mice following treatment with D-mannose or/and anti–PD-1 antibody was determined. Tumors were measured at the indicated time points. n = 8 mice per group. Statistical differences were determined by ordinary one-way ANOVA. ns, no significance, **** P < 0.0001. ( D ) Kaplan–Meier survival curves for mice injected with 4T1 cells and treated with D-mannose or/and anti–PD-1 antibody. The P value, comparing every two groups, was determined using log-rank test and is shown in the table Below the figure. ( E ) Western blot analysis of PD-L1 level in 4T1 tumor tissues as indicated. ( F ) Immunohistochemistry showing CD8 + T cell infiltration and granzyme B expression in the 4T1 tumor tissues as indicated (Scale bars, 100 μm). ( G ) Quantifications of images in ( F ). Data represent mean ± SD from six independent samples of each group. Statistical differences were determined by ordinary one-way ANOVA. ns, no significance, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: Mouse primary CD8 + T cells were activated with phorbol 12-myristate 13-acetate (PMA) (50 ng/mL, MedChemExpress) and ionomycin (500 ng/mL, MedChemExpress) for 12 h and then cocultured with 4T1 cells.

    Techniques: Injection, Western Blot, Immunohistochemistry, Expressing